2012;32(3-4):1517. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. 2020;9:113. 2014;119:2238. Eur J Pharmacol. J Proteome. Also, with those reported by Elabd et al. Ungefroren H, Davidoff M, Ivell R. Post-transcriptional block in oxytocin gene expression within the seminiferous tubules of the bovine testis. 2020;10(8):1268. https://doi.org/10.3390/ani10081268. Theriogenology. To the best of our knowledge, only a previous study, conducted in humans, evaluated the relationship of seminal OXT to male fertility and found that fertile men had lower SP-OXT concentrations than infertile men [27]. Manage cookies/Do not sell my data we use in the preference centre. 2014;5(1):4082. https://doi.org/10.1038/ncomms5082. Stillbirth numbers are typically higher in older parity sows and generally occur later in the birth order. Cyclopropane anesthesia may modify oxytocin's cardiovascular effects, so as to produce unexpected results such as hypotension. Mostafa et al. Sperm functional parameters were assessed in semen samples extended in the same Biosem+ extender used to prepare AI-doses, to a final concentration of 30106 sperm/mL. A stillbirth is defined as a piglet that is normally developed but dies shortly after or during parturition and does not breath. Cern JJ, Contreras-Aguilar MD, Escribano D, Martnez-Mir S, Lpez-Martnez MJ, Ortn-Bustillo A, Franco-Martnez L, Rubio CP, Muoz-Prieto A, Tvarijonaviciute A, Lpez-Arjona M, Martnez-Subiela S, Tecles F. BMC Vet Res. In Experiment 1, one-way ANOVA was used to investigate inter-breed, inter-boar variability on SP-OXT concentrations and intra-boar reliability was assessed by intra-class correlation (ICC) in a two-way mixed approach (ICC 3,1). https://doi.org/10.1016/j.theriogenology.2011.11.016. Oxytocin is frequently used to decrease farrowing time and birth interval as an aid to prevent stillbirths. Thereafter, 10L of biotinylated OXT-BSA (1 nmol/L, Cusabio Technology LLC, Houston, TX, USA) was added to each well and the plates incubated again at RT for 60min. Article CAS Exploring seminal plasma GSTM3 as a quality and in vivo fertility biomarker in pigsrelationship with sperm morphology. In Experiment 3, a multivariate statistical model [37] was applied to the in vivo fertility records in order to adjust them for parameters related to farm and sow and to identify the direct boar effect for farrowing rate and litter size. Seitchik J, Castillo M: Oxytocin augmentation of dysfunctional labor. Parrilla I, del Olmo D, Sijses L, Martinez-Alborcia MJ, Cuello C, Vazquez JM, et al. Overstimulation of the uterus by improper administration can be hazardous to both mother and fetus. https://doi.org/10.1111/j.1600-0897.2011.01033.x. Lets talk about it, and how it is used in labor induction. Milk ejection starts roughly 25 to 30 seconds later, however it only lasts for about 10 to 15 seconds. In pigs, the main role attributed to OXT is to facilitate the contractility of the myometrium [28]. 2015;50:1039. ; Data curation: L.P. and M.L-A. 2020;9:114. Electronically monitor the uterine activity and the fetal heart rate throughout the infusion of Pitocin. The control sows were allowed to farrow without intervention. J Androl. Epididymal oxytocin in the rat: its origin and regulation. Reprod Domest Anim. Biol Reprod. Percentage of litters from two farms with or without oxytocin administration, Table 1. Andrologia. Then, 15L of acceptor beads coated with monoclonal anti-oxytocin antibody (30g/mL) was added to each well and the plates were incubated at RT in darkness for 90min. Ejaculates with higher SP-OXT concentration/mL (hierarchically grouped; P<0.001) had larger volume and came from younger boars (P<0.05). Oxytocin is a hormone thats produced in the hypothalamus Moreover, variability among individuals in the SP-OXT has also been reported in humans [27]. J Perinat Educ. The influence of seminal plasma on offspring development and health. Following intravenous administration of oxytocin, uterine response occurs almost immediately and subsides within 1 hour. I. 2023 BioMed Central Ltd unless otherwise stated. Pitocin is indicated to produce uterine contractions during the third stage of labor and to control postpartum bleeding or hemorrhage. These differences were expected considering (1) variations in OXT concentrations have been reported when different OXT quantification methods are used [38], circumstance occurring among the three studies mentioned above, including the present report and (2) that differences between species in other SP components (proteins) had been previously reported and could due to species-related differences in mating strategy (vaginal vs uterine) [39]. 2018;53(S2):1801. The proved presence of OXTR along segments of the male reproductive tract of several mammalian species would confirm a local action of OXT [45, 46]. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. 2019;137:27. Improper oxytocin use can also cause an increased number of stillbirths by causing ruptured umbilical cords that lead to decreased oxygen delivery to the piglet during birth. Cochrane Database Syst Rev. Seitchik J, Amico J, et al: Oxytocin augmentation of dysfunctional labor. Labor stimulation with oxytocin: Effects on obstetrical and neonatal outcomes. Boars were free of chromosomal translocations and with percentages of spermatozoa with fragmented nuclear DNA lower than 3% (measured using the sperm chromatin stability assay, following the procedure described by Martinez-Pastor et al., [35]). https://doi.org/10.1016/j.jprot.2013.05.029. 1999;51(4):85565. None of the assessed sperm parameters differed between the two SP-OXT groups, neither those assessed at 0h nor those assessed at 72h of storage at 17C. 2021 Apr 1;17(1):140. doi: 10.1186/s12917-021-02838-5. Drugs.com provides accurate and independent information on more than 24,000 prescription drugs, over-the-counter medicines and natural products. Vet Q. Recuero S, Fernandez-Fuertes B, Bonet S, Barranco I, Yeste M. Potential of seminal plasma to improve the fertility of frozen-thawed boar spermatozoa. Consequently, OXT is sometimes used as an additive to semen AI-doses in order to accelerate the progression of semen towards the uterus for better colonization of the uterine-tubal reservoir [29]. L.P and M.L-A were financially supported by MINECO (BES-2016-076404) and MECD (FPU16/02170), Spain, respectively. It is indicated for (1) induction of labor in patients with a medical indication for the initiation of labor, such as Rh problems, maternal diabetes, preeclampsia at or near term, when delivery is in the best interests of mother and fetus or when membranes are prematurely ruptured and delivery is indicated; (2) stimulation or reinforcement of labor, as in selected cases of uterine inertia; (3) as adjunctive therapy in the management of incomplete or inevitable abortion. National Institutes of Health, Office of Research on Women's Health. The SP samples were stored in 3mL-cryotubes at 80C (Ultra Low Freezer; Haier Inc., Qingdao, China) until OXT measurement. Even with proper administration and adequate supervision, hypertonic contractions can occur in patients whose uteri are hypersensitive to oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. eCollection 2022. Maternal death due to oxytocin-induced water intoxication has been reported. Porcine seminal protein-I and II mRNA expression in boar spermatozoa is significantly correlated with fertility. https://doi.org/10.1016/j.tjem.2018.08.001. Indeed, recent studies have highlighted some SP proteins and peptides as putative biomarkers for sperm functional capacity and in vivo fertility outcomes of pig semen AI-doses [13,14,15,16]. Despite these considerations, there was a good reliability among ejaculates within boar, as ICC (3,1) was 0.78 (0.610.90; 95%). Seminal plasma proteins as potential markers of relative fertility in boars. The last studies in this realm have proposed searching for these biomarkers in seminal plasma (SP), a protein rich-fluid composed mainly by secretions from epididymis and accessory sex glands [8]. Some women have no problems with the medication, while others end up dissatisfied with the effect it has on their labor. WebPitocin is made from pregnant pig hormones. Intravenous infusion of 10 units of Pitocin added to 500 mL of a physiologic saline solution or 5% dextrose-in-water solution may help the uterus contract after a suction or sharp curettage for an incomplete, inevitable, or elective abortion. Thackare H, Nicholson HD, Whittington K. Oxytocin--its role in male reproduction and new potential therapeutic uses. https://doi.org/10.1093/humupd/dmk002. 1992;216(2):3113. Sperm membrane lipid disorders were assessed in 50L semen samples extended in 950L of PBS and incubated with 2.5L of H-42 (0.05mg/mL in PBS) and 10L of Yo-Pro-1 (2.5mol/L in DMSO) at 38C in darkness for 8min. Prostaglandin is one of the hormones that is produced naturally by the sow to end pregnancy and start the farrowing process. Table1 shows the median and the 2575th percentiles of boar age, ejaculate characteristics and sperm parameter assessed in each SP-OXT group. Oxytocin is a hormone produced in the hypothalamus and excreted by the pituitary gland. Prez-Patio C, Parrilla I, Barranco I, Vergara-Barbern M, Sim-Alfonso EF, Herrero-Martnez JM, et al. To the very best of our knowledge, this is apparently the first study measuring the concentration of OXT in pig SP and the first report conducted in a livestock species assessing an eventual putative influence of SP-OXT concentrations on sperm reproductive performance post-AI. https://doi.org/10.1016/j.theriogenology.2014.01.003. Violin plots displaying oxytocin (ng/mL) concentration levels and its distribution in seminal plasma (SP-OXT) of entire ejaculates (n=72) from 18 artificial insemination boars (four ejaculates per boar). [29] reported that the supplementation of pig semen AI-doses with OXT increased the number of sperm in the utero-tubal junction reservoir, having a positive impact on fertility. Results from non-normality distribution data were shown as median (25th and 75th percentiles), and those from normality distribution data as meanstandard error of the mean (SEM). https://doi.org/10.1111/j.1365-2605.1996.tb00476.x. Part of Epub 2020 Aug 31. Nagasawa T, Kimura Y, Masuda K, Uchiyama H. Animals (Basel). WebI'm always talking about being informed and we'll educated.not only is pitocin made from pig hormones and cervadil made from pig semen BUT..its not healthy or safe for mother or baby. Improvements in fertility, both in terms of farrowing rates and litter size, have been documented in sows inseminated with semen doses supplemented with OXT [30,31,32,33,34]. Oxytocin is the hormone that naturally induces labor. Pitocin Description. Pitocin is a nonapeptide found in pituitary extracts from mammals. It is standardized to contain 10 units of oxytocic hormone/mL and contains 0.5% Chlorobutanol, a chloroform derivative as a preservative, with the pH adjusted with acetic acid. Pitocin may contain up to 16% of total impurities. An oxytocic agent is any drug that can speed up the process of childbirth. Intravenous infusion (drip method) is the only acceptable method of parenteral administration of Pitocin for the induction or stimulation of labor. These properties are thought to be due to the fact that oxytocin and vasopressin differ in regard to only two of the eight amino acids (see PRECAUTIONS section). In pigs, an empirical study reported that supplementation of diluted semen with OXT did not influence sperm motility or the integrity of plasma and acrosomal membranes in both fresh and frozen-thawed sperm [29]. The protocol used in your case will depend on why you are being induced and other factors specific to your medical needs. Effects of different sexual stimuli on oxytocin release, uterine activity and receptive behavior in estrous sows. It is convenient to piggyback the Pitocin infusion on a physiologic electrolyte solution, permitting the Pitocin infusion to be stopped abruptly without interrupting the electrolyte infusion. Plasma concentrations of oxytocin and 13, 14-dihydro-15-keto-prostaglandin F2a in spontaneous and oxytocin-induced labor at term. To improve AI-efficiency, the latest trends point towards to a reduction in both the sperm numbers per AI-dose and the number of AIs performed per sow, which will lead to more than 6,000 sows being inseminated each year with semen from a single boar [4, 5]. Reproduction. https://doi.org/10.1016/j.semcdb.2019.06.008. An evaluation of the risk factors for stillbirths on two commercial swine farms in Brazil indicated that use of oxytocin increased the risk for stillbirth. The fluorescence intensity was measured through the EnSpire Multimode Plate Reader. 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